Research Group Dr. habil. Henrik Buschmann

Research leader / University lecturer (Privatdozent)

Botany Department
University of Osnabrück
Barbara Str. 11
49076 Osnabrück
phone 0049 541 9692248

My work covers three areas:

The evolution of cell division

There is something weird about plant cells: they are lacking centrosomes. This is true for all vascular plants - which is the vast majority of land plants. How then are they orchestrating cell division? In animals, centrosomes have a role in building the mitotic spindle and they are important for controlling the plane of cell division. These functions are also important for plants, so it seems reasonable to assume that some other entity (molecule, protein, structure?) has taken over the function of the centrosome.

This entity is likely the preprophase band (including its associated proteins). The preprophase band encircles the premitotic cell and outlines the future plane of cell division. We are investigating … 

Interested? Join our meeting in Seville, 2019 (organizers: H. Buschmann & A. Holzinger):

Plant cell expansion and handedness

Humans tend to confuse left and right occasionally. Plants don’t: Arabidopsis mutants like tortifolia1 (also called, convoluta or spiral2) are always right-handed. Precisely: all tortifolia1 organs show a right-handed twist when expanded. Why? Cortical microtubules of tortifolia1 are left-handed helical on average. This suggests that the cell wallmicrofibrils are left-handed too, which may explain right-handed growth. But what exactly makes the microtubules left-handed? We are investigating this question using single-molecule and superresolution microscopy.

Using super-fast growing plants for waste water clean-up (Symbiofilter)

In many developed countries drinking water quality suffers an alarming decline. The British Geological Survey (NERC) coined this the „nitrate time bomb“, because nitrate stored underground arrives at the groundwater level with considerable delay. High nitrate in drinking waters is dangerous for newborns. But also lakes and rivers suffer from nutrient overflow, resulting in algal blooms with concurrent impact on ecology and tourism. We are working on a solution to this problem by removing excess nutrient from waste waters through fast growing water cress (Lemna) cultures. This work involves a collaboration with a local company (Aqualight, Bramsche, Germany) and a local sewage plant (also in Bramsche).

Symbiofilter. Water cress, prototype, washer.

People involved in the recent projects
Henrik Buschmann (PI)
Agnes Borchers (PhD student)
Maike Bergmann (Research assistant)
Maike Lammers (Master student)
Johannes Konermann (Master student)
Ulrike Coja (Technician)

Collaborators (among others)
Aqualight (company, Bramsche, Germany)
Prof. emer. Hilmar Franke

Related publications by Henrik Buschmann (* indicates corresponding author)

Regensdorff, M., Deckena, M., Bergmann, M., Borchers, A., Scherer, G., Lammers, M., Hänsch, R., Zachgo, S., Buschmann, H.* 2018. Transient genetic transformation of Mougeotia scalaris (Zygnematophyceae) mediated by the endogenous α-tubulin1 promoter. Journal of Phycology. in press

Nishiyama, T. Sakayama, H., de Vries, J., Buschmann, H., Saint-Marcoux, D., Ullrich, K.K., Wilhelmsson, P.K.I., Vanderstraeten, L., Becker, D. et alii et Rensing, S.A. 2018. The Chara genome: secondary complexity and implications for plant terrestrialization. Cell. in press

Buschmann, H.* 2018. Myosin XI: Back on the scene – on the division machine. Insight Botany. Journal of Experimental Botany. in press

Borchers, A., Deckena, M., Buschmann, H.* 2018. Arabidopsis petiole torsions induced by lateral light or externally supplied auxin require microtubule-associated TORTIFOLIA1/SPIRAL2. Protoplasma in press

Mir, R., Morris, V., Buschmann, H., Rasmussen, C. 2017. Synthetic tangled1 auxin-induced-in-roots9 mutant phenotype used to assess TANGLED1 function. Plant Physiology 176: 418-431.

Smertenko, A., Assaad, F., Baluska, F., Bezanilla, M., Buschmann, H., Van Damme, D., Drakakaki, G., Hauser, et al., 2017. Plant cytokinesis: terminology for structures and processes. Trends in Cell Biology 27: 885-894.

Buschmann, H.* and Zachgo, S. 2016. The evolution of cell division: from streptophyte algae to land plants. Trends in Plant Science 21: 872–883.

Buschmann, H.* 2016. Plant cell division analyzed by transient Agrobacterium-mediated transformation of tobacco BY-2 cells. Book chapter in Methods in Molecular Biology: Plant Cell Division. Editor: Marie-Cecile Caillaud. 1370:17-25.

Buschmann, H.*, Holtmannspötter, M., Borchers, A., O´Donoghue, M-T., Zachgo, S. 2016. Microtubule dynamics of the centrosome-like polar organizers from the basal land plant Marchantia polymorpha. New Phytologist 209: 999-1013.

Derbyshire, P., Ménard, D., Green, P., Saalbach, G., Buschmann, H., Lloyd, C.W. and Pesquet, E. 2015. Proteomic analysis of microtubule-interacting proteins over the course of xylem vessel formation in Arabidopsis. The Plant Cell 10: 2709-2726.

Buschmann, H.*, Dols, J., Kopischke, S., Peña, E.J., Andrade-Navarro, M.A., Heinlein, M., Szymanski, D.B., Zachgo, S., Doonan,  J.H. and Lloyd, C.W. 2015. Arabidopsis KCBP interacts with AIR9 but remains in the cortical division zone throughout mitosis via its MyTH4-FERM domain. Journal of Cell Science 128:2033-2046.

Sambade, A., Findlay, K., Schäffner, A.R., Lloyd, C.W., Buschmann, H.* 2014. Actin-dependent and -independent functions of cortical microtubules in the differentiation of Arabidopsis leaf trichomes. Plant Cell 26: 1629-1644.

Peña, E.J., Ferriol, I., Sambade, A., Buschmann, H., Niehl, A., Elena, S.F., Rubio, L., Heinlein, M. 2014. Experimental virus evolution reveals a role of plant microtubule dynamics and TORTIFOLIA1/SPIRAL2 in RNA trafficking. PLoS One 9: e105364.

Sambade, A., Pratap, A., Buschmann, H., Morris, R., Lloyd, C.W. 2012. The influence of light on microtubule dynamics and alignment. Plant Cell 24: 192-201.

Buschmann, H.*, Green, P., Sambade, A., Doonan, J.H., Lloyd, C.W. 2011. Cytoskeletal dynamics in interphase, mitosis and cytokinesis analysed through Agrobacterium-mediated transient transformation of tobacco BY-2 cells. New Phytologist 190: 258–267.

Buschmann, H.* , Sambade, A., Pesquet, E., Calder, G., Lloyd, C.W. 2010. Microtubule dynamics in plant cells. Book chapter in Methods in Cell Biology, Editor: Lynne Cassimeris. Vol. 97: 373-400.

Buschmann, H., Hauptmann, M., Niessing, D., Lloyd, C.W. and Schäffner, A.R. 2009. Helical growth of Arabidopsis mutant tortifolia2 does not depend on cell division patterns but involves handed twisting of isolated cells. Plant Cell 21: 2090-2106.

Buschmann, H.* and Lloyd, C.W. 2008. Arabidopsis mutants and the network of microtubule-associated functions. Review. Molecular Plant 1: 888-898.

Lloyd, C.W. and Buschmann, H. 2007. Plant division: remembering where to build the wall. Review. Current Biology 17: 1053-1055.

Huska, M.R., Buschmann, H. and Andrade-Navarro, M.A. 2007. BiasViz: Visualization of amino acid biased regions in protein alignments. Bioinformatics 23: 3093-3094.

Korolev, A.V., Buschmann, H., Doonan, J.H. and Lloyd, C.W. 2007. AtMAP70-5, a divergent member of the MAP70 family of microtubule-associated proteins, is required for anisotropic cell growth in Arabidopsis. Journal of Cell Science 120: 2241-2247.

Buschmann, H.*, Sanchez-Pulido, L., Andrade-Navarro, M.A. and Lloyd, C.W. 2007. Homologues of Arabidopsis microtubule-associated AIR9 in trypanosomatid parasites: Hints on evolution and function. Plant Signaling & Behavior 2: 1-4.

Buschmann, H.*, Chan, J., Sanchez-Pulido, L., Andrade-Navarro, M.A., Doonan J.H., and Lloyd, C.W. 2006. Microtubule-associated AIR9 recognizes the cortical division site at preprophase and cell-plate insertion. Current Biology 16: 1938-1943.

Mao G., Buschmann H., Doonan J.H., and Lloyd C.W. 2006. The role of MAP65-1 in microtubule bundling during Zinnia tracheary element formation. Journal of Cell Science 119: 753-758.

Buschmann, H., Fabri, C.O., Hauptmann, M., Hutzler, P., Laux, T., Lloyd, C.W. and Schäffner, A.R. 2004. Helical Growth of the Arabidopsis mutant tortifolia1 reveals a plant-specific microtubule-associated protein. Current Biology 14: 1515-1521.

Buschmann, H. 2002. TORTIFOLIA Gene kontrollieren das Streckungswachstum in Arabidopsis. Klonierung von TOR1. Doctoral Dissertation.

Weise, A., Barker, L., Kühn, C., Lalonde, S., Buschmann, H., Frommer, W.B., and Ward, J.M. 2000. A new subfamily of sucrose transporters, SUT4, with low affinity/high capacity localized in enucleate sieve elements of plants. Plant Cell 12: 1345-1355.